Fig. 1

Validation of Cdkl5^-/y rats. (A) Schematic of the Cdkl5 knockout strategy depicting the WT and null alleles. The null allele has a 10 bp (bp) deletion in exon 8 (region shown in blue in WT allele), leading to a frame shift and an in frame, premature STOP codon forming in exon 9 (*). (B) Genotyping results from male WT and Cdkl5^−/y animals. Higher band in WT and Cdkl5^−/y animals resulting from F1 and R primers product. Lower band in the WT samples resulting from F2 and R primer products is absent in the null samples due to the 10 bp deleted sequence. (C) Western blot showing the absence of CDKL5 in hippocampal and prefrontal cortex tissue preparations from WT and Cdkl5^−/y rats. (D) Quantification of CDKL5 western blot protein expression in hippocampal and prefrontal cortex preparations. (E) Western blot showing the absence of CDKL5 in hippocampal synaptosome preparations from WT and Cdkl5^−/y rats. (F) Quantification of CDKL5 western blot protein expression in hippocampal synaptosome preparations