Fig. 5

Tph2 deficiency in rats is associated with changes in the clustering of subtypes of isolation-induced ultrasonic vocalizations. (A-L) Density plots depicting the distribution of individual isolation-induced ultrasonic vocalizations in Tph2^−/− (KO) and Tph2^+/− (HET) rat pups, as compared to Tph2^+/+ (WT) littermate controls, depending on nesting condition, i.e. WT-SN (A, G; ~ 35,000 calls), WT-CN (D, J; ~ 24,000 calls), HET-SN (B, H; ~ 34,000 calls), HET-CN (E, K; ~ 25,000 calls), KO-SN (C, I; ~ 20,000 calls), and KO-CN (F, L; ~ 14,000 calls). SN = standard nesting (blue), CN = communal nesting (orange). Color coding reflects frequencies as percentages. Individual isolation-induced ultrasonic vocalizations depicted here were recorded on postnatal day 12