Fig. 1
Downregulation of the Fmr1 gene in microglia. (A, B) The schematics illustrate the early and late developmental downregulation of the Fmr1 gene induced by either 4-OHT (50 mg/kg/i.p.) or tamoxifen (100 mg/kg/i.p.) administration, respectively. (C-F) Immunostaining against FMRP in microglia (Iba1+ cells) shows ablation of FMRP in microglia at early and late postnatal developmental stages in the corpus callosum of Fmr1 cKOearly and Fmr1 cKOlate compared to the controls (C, Female: Fmr1 cKOearly vs. control, t8 = 4.60, **p = 0.0018; D, Male: Fmr1 cKOearly vs. control, t8 = 2.81, *p = 0.0227, n = 5 for all groups) and Fmr1 cKOlate (E, Female: Fmr1 cKOlate vs. control, t7 = 3.54, **p = 0.0094; F, Male: Fmr1 cKOlate vs. control, t8 = 3.07, *p = 0.0154), n = 4–5). ImageJ was used to quantify the FMRP integrated density signal within the cell body of Iba1-positive cells in the corpus callosum, using maximum intensity projection images. Two sections per animal were imaged, and the integrated density of FMRP in 5–10 microglia per image was quantified. The values were averaged to obtain a single value per mouse. Unpaired two-tailed Student’s t-test. Data points represent individual mice. Data are shown as mean ± SEM. The scale bars are 25 μm. *p < 0.05 and **p < 0.01