Fig. 3
From: Autistic behavior is a common outcome of biallelic disruption of PDZD8 in humans and mice
Enhanced locomotor activity and metabolic rate in Pdzd8tm1b mice. A Locomotor activity on the cage floor (x–y beam breaks) was significantly different between Pdzd8tm1b mice (n = 9♂) and WT controls (n = 9♂) (two-way ANOVA: F = 8.292, p = 0.00705). Post-hoc pairwise corrected t-tests showed a significant difference only during the dark phase (t = 2.654, p = 0.035). B Locomotor activity on the cage floor (x–y beam breaks) throughout the 12-hour light/dark cycle over 96 h. C Locomotor activity (in-cage running wheel revolutions) was significantly different between Pdzd8tm1b mice and WT controls (two-way ANOVA: genotype: F = 12.441, p = 0.001294). Post-hoc pairwise corrected t-tests showed a significant difference only during the dark phase (t = 3.451, p = 0.0066). D Locomotor activity (in-cage running wheel revolutions) throughout the 12-hour light/dark cycle over 96 h. E Metabolic rate measured as mean hourly heat production was elevated in Pdzd8tm1b mice (two-way ANCOVA: genotype: F = 4.783, p = 0.036). For an equivalent 25 g mouse, the metabolic rate was significantly elevated during both the lights-on phase (post-hoc pairwise corrected t-test: t = 2.461, p = 0.026) and dark phase (post-hoc pairwise corrected t-test: t = 3.073, p = 0.0073). F Reduced body weight (g) of male Pdzd8tm1b mice versus WT controls at 11 weeks of age (unpaired t-test: t = 5.8603, p = 0.000026). G Unaltered food intake (g) in Pdzd8tm1b mice versus WT controls. H Unaltered respiratory exchange ratio in Pdzd8tm1b mice versus WT controls (two-way ANOVA: genotype: F = 0.269, p = 0.607). g, grams; MR, metabolic rate; RER, respiratory exchange ratio; revs, revolutions; tm1b, Pdzd8tm1b homozygous; WT, wild-type. *p < 0.05, **p < 0.01, ***p < 0.001 versus WT