Fig. 8
From: Autistic behavior is a common outcome of biallelic disruption of PDZD8 in humans and mice
Decreased adult neurogenesis in Pdzd8tm1b mice. A Number of EdU-positive cells relative to surface area (10 mm2) in sequential coronal sections of the hippocampus from Pdzd8tm1b mice (n = 55 sections from n = 5♀ mice) and WT controls (n = 70 sections from n = 5♀ mice) (Mann–Whitney: U = 1,286, p < 0.0014). B Representative images of EdU staining in the hippocampus of WT control (top) and Pdzd8tm1b mouse (bottom) with EdU-positive cells indicated by white arrows. The striations are artifacts caused by uneven illumination by the AxioScan Slide Scanner of the component image tiles that were assembled into the whole-section images. Scale bar: 200 μm. C Number of EdU-positive cells relative to surface area (mm2) in sequential coronal sections of the granule cell layer of the OB from Pdzd8tm1b mice (n = 54 sections from n = 5♀ mice) and WT controls (n = 73 sections from n = 5♀ mice) (Mann–Whitney: U = 1,307, p = 0.0011). D Number of EdU-positive cells relative to surface area (mm2) in sequential coronal sections of the extra granule cell layer of the OB from Pdzd8tm1b mice (n = 54 sections from n = 5♀ mice) and WT controls (n = 73 sections from n = 5♀ mice) (Mann–Whitney: U = 897, p < 0.0001). E Representative images of EdU staining in the OB of WT control (left) and Pdzd8tm1b mouse (right) with white box showing EdU-positive cells in a zoomed-in area. Scale bar: 400 μm. +, positive; OB, olfactory bulb; tm1b, Pdzd8tm1b homozygous; WT, wild-type