Fig. 2

Reduced intrinsic excitability of the SOM interneurons in the mPFC of SOM-Cre/Magel2 KO mice. A Cartoon showing the injection of AAV9-hSyn-DIO-ChR2-mCherry into mPFC. SOM interneurons of acute brain slices from SOM-Cre/Magel2 WT and SOM-Cre/Magel2 KO mice were recorded in a whole-cell configuration. B Representative traces comparing evoked firings in SOM neurons. C-F Quantitative analysis of resting membrane potential, half-width, current rheobase, and the mean amplitude of afterhyperpolarization. The current injection traces are displayed on the right of the current rheobase. Sample size of 14 in 6 mice of C, D, F was calculated. Sample size of 15 in 6 mice of E was determined. G SOM-expressing interneurons in the mPFC of SOM-Cre/Magel2 KO mice exhibited lower spike rates in response to current injections from 200 to 300 pA in steps of 20 pA (n = 12 cells in 6 mice per group). Results are presented as means ± SEM. Data in C, D, E, F were analyzed via unpaired t-test. Data in (G) are statistically compared by two-way ANOVA with Tukey’s post hoc test. **p < 0.01,***p < 0.001, compared to the control if not designated. “ns” indicating no statistical significance