Fig. 3

mPFC SOM interneurons are blunted activated during social interaction in SOM-Cre/Magel2 KO mice. A Schematic diagram of the fiber photometry setup. Calcium transients were recorded from GCaMP7s-positive SOM interneurons of mice subjected to the three-chamber social interaction task. B-C Viral strategy (B) and histological verification (C) for fiber photometry recordings of mPFC SOM interneurons. D-E GCaMP7s signals of SOM interneurons during the bouts of social interaction with novel object (light blue) or novel mouse (pink) in the three-chamber test. Calcium signals of a SOM-Cre/Magel2 WT mouse showed the minimal increase to interact with the novel object. F The heatmap display of calcium signals was aligned to the start of individual interactions. Note that time 0 was considered as the time point when the experimental animal was closest to the novel object. Each row indicates one bout, and a total of eight bouts are shown. H Representative line of the peri-event plot of the averaged calcium signals. G, I The same as F, H but for novel mouse interaction. J-M Heat maps (J, K) and per-bout stacked plots (L, M) of SOM-GCaMP7s signals were the same as F and G but for SOM-Cre/Magel2 KO. O, P Statistical comparison of fluorescence signals (O: Area under the curve; P: Peak amplitude) of mPFC SOM interneurons between novel object interaction and novel mouse interaction. All results are presented as means ± SEM. n = 10 animals per group. ^***p < 0.001 for novel mouse vs. novel object