Table 1 Collated table of statistical analysis
Genotype | Mean ± SEM | n = # of synaptosomes or coverslips/ N = # of neuronal preparations | Comparison | P | Statistical test | |
|---|---|---|---|---|---|---|
Figure 2 | ||||||
Figure 2C | WT | 1.000 ± 0.152 | 3 | WT vs. Nrxn1+/− | 0.0365 | Unpaired t test |
Nrxn1+/− | 0.445 ± 0.096 | 3 | ||||
Figure 2E | WT | 16.65 ± 1.76 | 9/3 | WT vs. Nrxn1+/− | 0.759 | |
Nrxn1+/− | 15.91 ± 1.58 | |||||
Figure 2F | WT | 0.408 ± 0.045 | 9/3 | WT vs. Nrxn1+/− | 0.716 | |
Nrxn1+/− | 0.383 ± 0.048 | |||||
Figure 2G | WT | 0.301 ± 0.046 | 9/3 | WT vs. Nrxn1+/− | 0.675 | |
Nrxn1+/− | 0.276 ± 0.036 | |||||
Figure 2I | WT | 44.83 ± 4.68 | 9/3 | WT vs. Nrxn1+/− | 0.822 | |
Nrxn1+/− | 46.46 ± 5.40 | |||||
Figure 2J | WT | 46.69 ± 4.42 | 9/3 | WT vs. Nrxn1+/− | 0.191 | |
Nrxn1+/− | 58.69 ± 7.59 | |||||
Figure 2L | WT | 100 ± 8.91 | 13/3 | WT vs. Nrxn1+/− | 0.0037 | |
Nrxn1+/− | 60.12 ± 8.61 | |||||
Figure 2N | WT | 100 ± 9.46 | 12/3 | WT vs. Nrxn1+/− | 0.761 | |
Nrxn1+/− | 104.00 ± 8.93 | 15/3 | ||||
Figure 3 | ||||||
Figure 3C | WT | 1.000 ± 0.086 | 4 | WT vs. Nlgn−/y | < 0.0001 | Unpaired t test |
Nlgn3−/y | 0.025 ± 0.008 | 4 | ||||
Figure 3E | WT | 15.65 ± 2.03 | 11/3 | WT vs. Nlgn−/y | 0.883 | Mann-Whitney test |
Nlgn3−/y | 15.91 ± 2.36 | 14/3 | ||||
Figure 3F | WT | 0.478 ± 0.049 | 11/3 | WT vs. Nlgn−/y | 0.841 | |
Nlgn3−/y | 0.416 ± 0.036 | 14/3 | ||||
Figure 3G | WT | 0.376 ± 0.043 | 11/3 | WT vs. Nlgn−/y | 0.418 | Unpaired t test |
Nlgn3−/y | 0.332 ± 0.034 | 14/3 | ||||
Figure 3I | WT | 52.81 ± 4.90 | 11/3 | WT vs. Nlgn−/y | 0.582 | Mann-Whitney test |
Nlgn3−/y | 59.29 ± 6.24 | 14/3 | ||||
Figure 3J | WT | 55.81 ± 5.81 | 11/3 | WT vs. Nlgn−/y | 0.051 | |
Nlgn3−/y | 37.24 ± 2.79 | 14/3 | ||||
Figure 3L | WT | 100 ± 7.10 | 11/3 | WT vs. Nlgn−/y | 0.0112 | Unpaired t test |
Nlgn3−/y | 76.65 ± 4.73 | 12/3 | ||||
Figure 3N | WT | 100 ± 8.55 | 14/4 | WT vs. Nlgn−/y | 0.183 | |
Nlgn3−/y | 83.97 ± 8.05 | 15/4 | ||||
Figure 4 | ||||||
Figure 4B | WT | 10.35 ± 0.71 | 12/3 | WT vs. Syngap+/− | 0.620 | One-way ANOVA with Dunnett’s multiple comparisons test |
Syngap+/− | 9.13 ± 1.34 | 11/3 | WT vs. Syngap−/− | 0.933 | ||
Syngap−/− | 9.91 ± 0.99 | 12/3 | ||||
Figure 4C | WT | 0.461 ± 0.030 | 12/3 | WT vs. Syngap+/− | 0.218 | |
Syngap+/− | 0.524 ± 0.029 | 11/3 | WT vs. Syngap−/− | 0.725 | ||
Syngap−/− | 0.488 ± 0.025 | 12/3 | ||||
Figure 4D | WT | 0.375 ± 0.024 | 12/3 | WT vs. Syngap+/− | 0.753 | |
Syngap+/− | 0.401 ± 0.028 | 11/3 | WT vs. Syngap−/− | 0.338 | ||
Syngap−/− | 0.427 ± 0.033 | 12/3 | ||||
Figure 4F | WT | 46.61 ± 4.89 | 12/3 | WT vs. Syngap+/− | > 0.999 | Kruskal-Wallis with Dunn’s multiple comparisons test |
Syngap+/− | 42.98 ± 4.76 | 11/3 | WT vs. Syngap−/− | 0.828 | ||
Syngap−/− | 43.08 ± 5.47 | 12/3 | ||||
Figure 4G | WT | 51.01 ± 5.11 | 12/3 | WT vs. Syngap+/− | 0.886 | |
Syngap+/− | 48.27 ± 7.00 | 11/3 | WT vs. Syngap−/− | > 0.999 | ||
Syngap−/− | 48.39 ± 6.73 | 12/3 | ||||
Figure 4I | WT | 100 ± 4.07 | 15/3 | WT vs. Syngap+/− | 0.171 | One-way ANOVA with Tukey’s multiple comparisons test |
Syngap+/− | 83.98 ± 7.93 | 16/3 | WT vs. Syngap−/− | 0.007 | ||
Syngap−/− | 70.80 ± 7.34 | 16/3 | ||||
Figure 4K | WT | 100 ± 7.61 | 15/3 | WT vs. Syngap+/− | 0.980 | |
Syngap+/− | 101.9 ± 6.29 | 15/3 | WT vs. Syngap−/− | 0.210 | ||
Syngap−/− | 118.9 ± 11.17 | 13/3 | ||||
Figure 5 | ||||||
Figure 5B | WT | 13.74 ± 1.72 | 14/3 | WT vs. Syngap+/Δ−GAP | 0.672 | One-way ANOVA with Dunnett’s multiple comparisons test |
Syngap+/Δ−GAP | 16.09 ± 2.90 | 12/3 | WT vs. SyngapΔ − GAP/Δ−GAP | 0.966 | ||
SyngapΔ − GAP/Δ−GAP | 14.45 ± 2.11 | 10/3 | ||||
Figure 5C | WT | 0.443 ± 0.038 | 14/3 | WT vs. Syngap+/Δ−GAP | 0.454 | |
Syngap+/Δ−GAP | 0.504 ± 0.046 | 12/3 | WT vs. SyngapΔ − GAP/Δ−GAP | 0.956 | ||
SyngapΔ − GAP/Δ−GAP | 0.429 ± 0.034 | 10/3 | ||||
Figure 5D | WT | 0.392 ± 0.038 | 14/3 | WT vs. Syngap+/Δ−GAP | 0.882 | |
Syngap+/Δ−GAP | 0.415 ± 0.045 | 12/3 | WT vs. SyngapΔ − GAP/Δ−GAP | 0.445 | ||
SyngapΔ − GAP/Δ−GAP | 0.327 ± 0.037 | 10/3 | ||||
Figure 5F | WT | 33.55 ± 3.35 | 14/3 | WT vs. Syngap+/Δ−GAP | 0.696 | |
Syngap+/Δ−GAP | 37.51 ± 5.00 | 12/3 | WT vs. SyngapΔ − GAP/Δ−GAP | 0.179 | ||
SyngapΔ − GAP/Δ−GAP | 43.27 ± 3.54 | 10/3 | ||||
Figure 5G | WT | 32.74 ± 3.85 | 14/3 | WT vs. Syngap+/Δ−GAP | > 0.999 | Kruskal-Wallis with Dunn’s multiple comparisons test |
Syngap+/Δ−GAP | 34.96 ± 3.53 | 12/3 | WT vs. SyngapΔ − GAP/Δ−GAP | 0.785 | ||
SyngapΔ − GAP/Δ−GAP | 35.98 ± 3.00 | 10/3 | ||||
Figure 5I | WT | 100 ± 3.63 | 8/3 | WT vs. Syngap+/Δ−GAP | 0.135 | One-way ANOVA with Dunnett’s multiple comparisons test |
Syngap+/Δ−GAP | 74.91 ± 11.88 | 9/3 | WT vs. SyngapΔ − GAP/Δ−GAP | 0.012 | ||
SyngapΔ − GAP/Δ−GAP | 59.32 ± 9.84 | 9/3 | ||||
Figure 5K | WT | 100 ± 8.69 | 10/3 | WT vs. Syngap+/Δ−GAP | 0.763 | |
Syngap+/Δ−GAP | 107.3 ± 7.63 | 11/3 | WT vs. SyngapΔ − GAP/Δ−GAP | 0.655 | ||
SyngapΔ − GAP/Δ−GAP | 108.9 ± 7.87 | 13/3 | ||||
Figure 6 | ||||||
Figure 6C | WT | 1.000 ± 0.096 | 5 | WT vs. Pten+/− | 0.0178 | Unpaired t test |
Pten+/− | 0.676 ± 0.051 | 5 | ||||
Figure 6E | WT | 20.20 ± 2.34 | 15/4 | WT vs. Pten+/− | 0.639 | |
Pten+/− | 18.94 ± 1.41 | 19/4 | ||||
Figure 6F | WT | 0.396 ± 0.043 | 15/4 | WT vs. Pten+/− | 0.230 | |
Pten+/− | 0.457 ± 0.028 | 19/4 | ||||
Figure 6G | WT | 0.387 ± 0.032 | 15/4 | WT vs. Pten+/− | 0.329 | |
Pten+/− | 0.426 ± 0.025 | 19/4 | ||||
Figure 6I | WT | 36.06 ± 3.41 | 15/4 | WT vs. Pten+/− | 0.464 | |
Pten+/− | 39.04 ± 2.35 | 19/4 | ||||
Figure 6J | WT | 35.46 ± 3.99 | 15/4 | WT vs. Pten+/− | 0.308 | |
Pten+/− | 41.07 ± 3.64 | 19/4 | ||||
Figure 6L | WT | 100 ± 5.61 | 24/4 | WT vs. Pten+/− | 0.026 | |
Pten+/− | 79.96 ± 6.75 | 20/4 | ||||